LOCUS       pLV-[hsa-miR-513a-2]        9506 bp    DNA     circular     2-MAR-2011
SOURCE      
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7427..8287
                     /label=Amp(R)
     promoter        8488..9028
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             6032..6266
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6841..7296)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    6338..6468
                     /label=SV40 pA
     misc_feature    6469..6682
                     /label=SV40 ori
     misc_feature    5353..5949
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3971
                     /label=intron
     CDS             4018..5343
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3761
                     /label=hsa-mir-513a-2 precursor
BASE COUNT     2279 a      2436 c      2579 g      2212 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccg aaatgttata 
     3121 ttctggctaa gcctaaaatt tgtgtgttaa ttagccattc gcggtggtat gtgcctttaa 
     3181 tcccagctac ttgggaggct gaggcaggag aagtgcttag acacagaagg gggaggttgc 
     3241 agtgagccga gatcacacca atgcatgcca gtctgggcga cagagccaga cttcatctga 
     3301 aaacaaaaac aaaaacaaaa caaaaatctg catgttaaaa tctcacttgg ggcaggatga 
     3361 aaaggggagc gccgggggct acaagttgca ttgtcccttg gcataaatct tggtggccat 
     3421 gccaaggcat acacctatag ggacttgaaa aaaaacggca tgaataggga gcatttgttc 
     3481 ttggatgcca cattcagcca ttcagtgtgc agtgcctttc acagggaggt gtcatttatg 
     3541 tgaactaaaa tataaatttc acctttctga gaagggtaat gtacagcatg cactgcatat 
     3601 gtggtgtccc tgaggatggg ggtgtagctg cgagcatggt gatgcctgtt ttgcatcctg 
     3661 tgcacccact cttcagtgct gggctgcttc agggaacttt ttgtgccttc taaatgacta 
     3721 tacataggaa ctgtggactc ctgagaaaac gcatgcactt agctagctcg agcttttgga 
     3781 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt 
     3841 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc 
     3901 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc 
     3961 ttccatttca ggtgtcgtga aaactacccc tctagagtcg agctaccggt cgccaccatg 
     4021 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg 
     4081 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc 
     4141 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc 
     4201 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc 
     4261 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg 
     4321 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc 
     4381 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg 
     4441 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc 
     4501 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag 
     4561 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac 
     4621 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc 
     4681 gccgagggcc gccactccac cggcggcatg gacgagctgt acaaggaccc accggtcgcc 
     4741 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 
     4801 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 
     4861 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 
     4921 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 
     4981 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 
     5041 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 
     5101 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 
     5161 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 
     5221 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 
     5281 gccgacgtcg agtgcccgaa ggaccgcgcg acctggtgca tgacccgcaa gcccggtgcc 
     5341 tgagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta 
     5401 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc 
     5461 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt 
     5521 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg 
     5581 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt 
     5641 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct 
     5701 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt 
     5761 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct 
     5821 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc 
     5881 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct 
     5941 ccccgcctgg aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga 
     6001 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag 
     6061 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga 
     6121 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct 
     6181 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt 
     6241 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt 
     6301 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa 
     6361 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca 
     6421 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc 
     6481 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 
     6541 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 
     6601 ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata 
     6661 gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc 
     6721 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata 
     6781 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg 
     6841 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     6901 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     6961 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 
     7021 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     7081 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     7141 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     7201 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     7261 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt 
     7321 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
     7381 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 
     7441 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 
     7501 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 
     7561 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 
     7621 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg 
     7681 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 
     7741 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 
     7801 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 
     7861 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 
     7921 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc 
     7981 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 
     8041 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 
     8101 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 
     8161 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 
     8221 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 
     8281 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt 
     8341 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 
     8401 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 
     8461 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 
     8521 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 
     8581 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa 
     8641 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 
     8701 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 
     8761 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 
     8821 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 
     8881 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 
     8941 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 
     9001 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 
     9061 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 
     9121 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg 
     9181 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg 
     9241 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 
     9301 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc 
     9361 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 
     9421 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca 
     9481 ctaaagggaa caaaagctgg agctgc 
//
